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螞蟻淘/Cell Counting Kit-8（CCK-8）細(xì)胞增殖檢測試劑盒/3000T/C6005L
  • 螞蟻淘/Cell Counting Kit-8（CCK-8）細(xì)胞增殖檢測試劑盒/3000T/C6005L

螞蟻淘/Cell Counting Kit-8（CCK-8）細(xì)胞增殖檢測試劑盒/3000T/C6005L

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貨號(hào): C6005L
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    • 產(chǎn)品概述：儲(chǔ)存條件4℃避光保存，長期儲(chǔ)存置于-20℃，有效期見外包裝。

      產(chǎn)品貨號(hào)

      C6005S

      C6005M

      C6005L

      C6005XL

      C6005XXL

      產(chǎn)品規(guī)格

      100T

      500T

      3000T

      10000T

      5×10000T

      組分

      1 mL

      5 mL

      30 mL

      100 mL

      5×100 mL

      產(chǎn)品介紹Cell Counting Kit-8 簡稱 CCK8（或 WST-8）試劑盒，是一種基于 WST-8（化學(xué)名：2-(2-甲氧基-4-硝苯基)-3-(4- 硝苯基)-5-(2,4-二磺基苯)-2H-四唑單鈉鹽）顯色反應(yīng)的廣泛應(yīng)用于細(xì)胞增殖和細(xì)胞毒性的快速高靈敏度檢測試劑盒。WST-8 工作原理：在電子耦合試劑存在的條件下，可以被線粒體內(nèi)的脫氫酶還原生成高度水溶性的橙黃色的甲臜產(chǎn)物（formazan）。顏色的深淺與細(xì)胞的增殖成正比，與細(xì)胞毒性成反比。使用酶標(biāo)儀在 450 nm 波長處測定 OD 值，間接反映活細(xì)胞數(shù)量。WST-8 是 MTT 的一種升級(jí)替代產(chǎn)品，和 MTT 或其它MTT 類似產(chǎn)品如 XTT、MTS 等相比有明顯的優(yōu)點(diǎn)。首先，MTT 被線粒體內(nèi)的一些脫氫酶還原生成的甲臜不是水溶性的，需要有特定的溶解液來溶解；而 WST-8、XTT 和 MTS產(chǎn)生的甲臜都是水溶性的，可以省去后續(xù)的溶解步驟。其次，WST-8 產(chǎn)生的甲臜比 XTT 和 MTS 產(chǎn)生的甲臜更易溶解。再次，WST-8 比 XTT 和 MTS 更加穩(wěn)定，使實(shí)驗(yàn)結(jié)果更加穩(wěn)定。另外，WST-8 與 MTT、XTT 相比線性范圍更寬，靈敏度更高。CCK 法廣泛應(yīng)用于藥物篩選、細(xì)胞增殖測定、細(xì)胞毒性測定、腫瘤藥敏試驗(yàn)以及生物因子的活性檢測等方面。
      說明書：UE-C6005S/C6005M/C6005L/C6005XL/C6005XXL
      MSDS：MSDS C6005Cell Counting Kit-8（CCK-8）
      客戶使用反饋：
      常見問題解答：◆為什么測的吸光度值過低？

      1.細(xì)胞數(shù)量過低，建議增加細(xì)胞待測數(shù)量；2.染色難度大，建議增加CCK-8孵育時(shí)間◆數(shù)據(jù)重復(fù)性差？1.孔板最外圈試劑容易揮發(fā)，建議最外一圈的孔只加培養(yǎng)基，不作為測定孔用；2.CCK-8沾壁，建議加入CCK-8后，輕輕敲擊培養(yǎng)板以幫助混勻；3.細(xì)胞數(shù)量過多或過少，建議預(yù)先在1000-100000個(gè)/孔范圍內(nèi)摸索條件；4.孔內(nèi)氣泡干擾酶標(biāo)儀檢測，檢測前需確保每個(gè)孔內(nèi)無氣泡◆在做細(xì)胞的加藥實(shí)驗(yàn)時(shí),藥物對(duì)CCK-8測定是否有影響?1.注意如果藥物具有還原性(如維生素C),會(huì)和CCK-8發(fā)生顯色反應(yīng),增加吸光度；2.藥物中的金屬對(duì)CCK-8顯色有影響:當(dāng)終濃度為1mM的氯化亞鉛、氧化鐵、硫酸銅會(huì)抑制5%,15%, 90%的顯色反應(yīng),使靈敏度降級(jí)。如果終濃度是10mM的話，將會(huì)100%抑制。解決辦法:首先要確認(rèn)藥物是否有吸收,在含有藥物的培養(yǎng)基中加入CCK-8,測定450nm的吸光度,如果它的吸光度比不含藥物的培養(yǎng)基(加CCK-8)的吸光度高,則證明藥物有影響,可在加CCK-8之前更換培養(yǎng)基,去掉藥物的影響；3.由于培養(yǎng)基中可能含有氧化還原反應(yīng)的物質(zhì),在正式實(shí)驗(yàn)之前,建議使用一個(gè)孔作一下檢測,有必要先確認(rèn)培養(yǎng)基和CCK-8是否反應(yīng)，一般正常的OD值應(yīng)該在0.4以下◆可否采用CCK-8法進(jìn)行藥物的IC50檢測？可以,將細(xì)胞培養(yǎng)后按照不同濃度的藥物處理一定時(shí)間后進(jìn)行CCK-8檢測,根據(jù)吸光度繪制曲線,計(jì)算該藥物抑制細(xì)胞數(shù)量一半時(shí)的濃度(IC50)◆CCK-8對(duì)于不同的細(xì)胞,靈敏度是否一樣？不一樣,懸浮細(xì)胞較貼壁細(xì)胞難染色。對(duì)于貼壁細(xì)胞,一般加入CCK-8培養(yǎng)1-4小時(shí)吸光度已經(jīng)很高,但對(duì)于懸浮細(xì)胞則可能吸光度較低,可以通過延長CCK-8的加入時(shí)間或增加細(xì)胞數(shù)量來解決◆若是暫不檢測OD值，該如何處理?若暫時(shí)不測定 OD 值，可以向每孔中加入 10μL 0.1 M 的 HCl 溶液或者 1% w/v SDS 溶液，并遮蓋培養(yǎng)板避光保存在室溫條件下。24 小時(shí)內(nèi)測定，吸光度不會(huì)發(fā)生變化◆用CCK-8測細(xì)胞活力時(shí)每次都要做標(biāo)準(zhǔn)曲線嗎？建議每次做，雖然細(xì)胞是一樣的，但是細(xì)胞的狀態(tài)不一定一樣。對(duì)于狀態(tài)不一樣的細(xì)胞，建議每次做標(biāo)準(zhǔn)曲線

      使用本產(chǎn)品的文獻(xiàn)：1.Oncogene miR-187-5p is associated with cellular proliferation, migration,invasion, apoptosis and an increased risk of recurrence in bladder cancer ZuweiLi ,CanbinLin,LiwenZhao,LiangZhou,XiangPan,JingQuan,XiqiPeng,WeiqingLi,HangLi,JinlingXu,WeijieXu,XinGuan,YunChen Biomedicine & Pharmacotherapy(2018) 應(yīng)用方向：細(xì)胞活性檢測：膀胱癌細(xì)胞（BC） 2.MicroRNA?572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis

    • Xiang Pan, Zuwei Li , Liwen Zhao, Jing Quan, Liang Zhou, Jinling Xu, Weiji Xu, Xin Guan, Hang Li , Shangqi Yang, Yaoting Gui Oncology Reports(2018) 3092-3101 應(yīng)用方向：細(xì)胞活性檢測：腎細(xì)胞（RCC）
      • 3.Oncogene miR-154-5p regulates cellular function and acts as a molecular marker with poor prognosis in renal cell carcinomaLin Canbin,Li Zuwei,Chen Peijie,Quan Jing,Pan Xiang,Zhao Liwen,Zhou Liang,Lai Yulin,He Tao,Xu Weijie,Xu Jinling,Guan Xin,Li Hang,Yang Shangqi,Hu YiminLife Sciences(2018)應(yīng)用方向：細(xì)胞活性檢測：膀胱癌細(xì)胞（BC）4.外泌體介導(dǎo)乳腺癌 MCF-7 細(xì)胞對(duì)多柔比星耐藥性的機(jī)制張如月； 李朵璐； 楊哲； 郭金秀； 周玉冰；藥學(xué)學(xué)報(bào)(2019)應(yīng)用方向：細(xì)胞活性檢測：MCF-7、MCF-7/Exo、MCF-7/Dox細(xì)胞5.MS-275聯(lián)合順鉑對(duì)食管鱗狀細(xì)胞癌9706細(xì)胞氧化損傷和凋亡的影響劉騰飛,王亞蘋,李亞,張振坤,李喆,周馨魁,張璐玉,王文杰,劉紅濤,張彥婷,關(guān)方霞,馬珊珊鄭州大學(xué)生命科學(xué)學(xué)院應(yīng)用方向：細(xì)胞的存活率檢測：食管鱗狀細(xì)胞癌(鱗癌) EC9706細(xì)胞6.A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in ratsMinghao Yao, Feng Gao, Ru Xu, Junni Zhang, Yihao Chena , Fangxia GuanBiomaterials Science應(yīng)用方向：細(xì)胞活性檢測：BMSC7.重組人MG53蛋白激活A(yù)kt/GSK-3β通路降低LPS誘導(dǎo)的hUC-MSCs氧化損傷王亞蘋 張振坤 李喆 劉騰飛 黃團(tuán)結(jié) 周馨魁 麻建杰 關(guān)方霞 馬珊珊鄭州大學(xué)學(xué)報(bào)（醫(yī)學(xué)版） 應(yīng)用方向：細(xì)胞活力檢測：人臍帶間充質(zhì)干細(xì)胞(hUC-MSCs) 8.Inonotus Obliquus Polysaccharides Induces Apoptosis of Lung Cancer Cells and Alters Energy Metabolism via the LKB1/AMPK Axis Shuping Jiang，F(xiàn)uli Shi，Hui Lin，Ying Ying，Lingyu Luo，Deqiang Huang，Zhijun Luo International Journal of Biological Macromolecules Volume 151, 15 May 2020, Pages 1277-1286 9.A chitosan-based thermosensitive scaffold loaded with bone marrow-derived mesenchymal stem cells promotes motor function recovery in spinal cord injured mice Junni Zhang,Tian Cheng,Yihao Chen,Feng Gao,Fangxia Guan,Minghao Yao Biomedical materials 10.Structure analysis of polysaccharides purified from Cyclocarya paliurus with DEAE-Cellulose and its antioxidant activity in RAW264.7 cells QiAn，XimeiYe，YiHan，MengZhao，SiChen，XinLiu，XiangLi，ZitongZhao，YangZhang，KehuiOuyang，WenjunWang International Journal of Biological Macromolecules(Available online 28 November 2019) 11.Dual-enzymatically crosslinked hyaluronic acid hydrogel as a longtime 3D stem cell culture system Feng Gao,Jinrui Li,Luyu Wang,Dan Zhang,Junni Zhang,Fangxia Guan，Minghao Yao Biomedical Materials 12.Enhanced effect of nano-monetite hydrosol on dentin remineralization and tubule occlusion Shenglong Tan，Shangsi Chen，Yifan Wang，F(xiàn)eige Wu，Yufei Shi，Jianglin Wang，Yinyin Du，Shengmin Zhang Dental Materials（Volume 36, Issue 6, June 2020, Pages 816-825） 13.Control the fate of human umbilical cord mesenchymal stem cells with dual-enzymatically cross-linked gelatin hydrogels for potential applications in nerve regeneration JinruiLi，F(xiàn)engGao，ShanshanMa，YantingZhang，JunniZhang，F(xiàn)angxiaGuan，MinghaoYao Journal of Tissue Engineering and Regenerative Medicine 14.MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis Shanshan Ma,Yaping Wang,Xinkui Zhou,Zhe Li,Zhenkun Zhang,Yingying Wang,Tuanjie Huang,Yanting Zhang,Jijing Shi,Fangxia Guan ACS Chem. Neurosci. 2020, 11, 17, 2590–2601 15.Sodium alginate/collagen hydrogel loaded with human umbilical cord mesenchymal stem cells promotes wound healing and skin remodeling Zhenkun Zhang, Zhe Li, Ya Li, Yingying Wang, Minghao Yao, Kun Zhang, Zhenyu Chen, Han Yue, Jijing Shi, Fangxia Guan & Shanshan Ma Cell and Tissue Research16.Reshaping the Immune Microenvironment by Oncolytic Herpes Simplex Virus in Murine Pancreatic Ductal AdenocarcinomaLiming Zhang，Wei Wang，Ruikun Wang，Jie Yin，Hongkai Zhang，Youjia CaoMolecular Therapy（VOLUME 29, ISSUE 2, P744-761, FEBRUARY 03, 2021）17.Selective extracellular arginine deprivation by a single injection of cellular non-uptake arginine deiminase nanocapsules for sustained tumor inhibitionHongzhao Qi,Yin Wang,Xubo Yuan,Peifeng Lia，Lijun YangNanoscale18.Inducing Apoptosis and Suppressing Inflammatory Reactions in Synovial Fibroblasts are Two Important Ways for Guizhi-Shaoyao-Zhimu Decoction Against Rheumatoid ArthritisZhang Q, Duan HX, Li RL, Sun JY, Liu J, Peng W, Wu CJ, Gao YXJournal of Inflammation Research（26 January 2021 Volume 2021:14 Pages 217—236） 19.Injectable bone cement with magnesium-containing microspheres enhances osteogenesis via anti-inflammatory immunoregulation Shenglong Tan，Yifan Wang，Yingying Du，Yin Xiao，Shengmin Zhang Bioactive Materials Volume 6, Issue 10, October 2021, Pages 3411-3423 20.Propionate and butyrate produced by gut microbiota after probiotic supplementation attenuate lung metastasis of melanoma cells in mice Lili Chen,Xinyu Zhou,Yawei Wang,Dake Wang,Yueshuang Ke,Xianlu Zeng Molecular Nutrition & Food Research 21.Regulating the Anticancer Efficacy of Sgc8-Combretastatin A4 Conjugates: A Case of Recognizing the Significance of Linker Chemistry for the Design ofAptamer-Based Targeted Drug Delivery Strategies Zhiyong Huang,Dan Wang,Cheng-Yu Long,Shen-Huan Li,Xue-Qiang Wang,Weihong Tan Journal of American Chemical Society

      22.Biomimetic Nanoparticles Carrying Repolarization Agent of Tumor-Associated Macrophages for Remodeling of the Inflammatory Microenvironment Following Photothermal TherapyYale Yue,Fenfen Li,Yao Li,Yazhou Wang,Xinjing Guo,Zhaoxia Cheng,Nan Li,Xiaotu Ma,Guangjun Nie,Xiao ZhaoACS Nano

      23.Taxifolin, an Inhibitor of Sortase A, Interferes With the Adhesion of Methicillin-Resistant Staphylococcal aureusLi Wang,Guangming Wang,Han Qu,Kai Wang,Shisong Jing,Shuhan Guan,Liyan Su,Qianxue Li,Dacheng WangSuggest a Research Topic 24.Targeted Mitochondrial Fluorescence Imaging-Guided TumorAntimetabolic Therapy with the Imprinted Polymer NanomedicineCapable of Specifically Recognizing Dihydrofolate Reductase

      Ya-Ting Qin,Yao-Jia Ma,Yu-Sheng Feng,Xi-Wen He,Wen-You Li,Yu-Kui Zhang

      ACS Applied Materials & Interfaces 25.PDGF-BB/SA/Dex injectable hydrogels accelerate BMSC-mediated functional full thickness skin wound repair by promoting angiogenesis Zhenkun Zhang,Zhe Li,Yingying Wang,Qianqian Wang,Minghao Yao,Liang Zhao,Jijing Shi,Fangxia Guan,Shanshan Ma Joural of Materials Chemistory B 26.Molecular subtypes based on CNVs related gene signatures identify candidate prognostic biomarkers in lung adenocarcinomaBaihui Li,Ziqi Huang,Wenwen Yu,Shaochuan Liu,Jian Zhang,Qingqing Wang,Lei Wu,Fan Kou,Lili YangNeoplasia Volume23,Issue 7,July 2021,Page 704-717 27.Injectable bone cement with magnesium-containing microspheres enhances osteogenesis via anti-inflammatory immunoregulationShenglong Tan,Yifan Wang,Yingying Du,Yin Xiao,Shengmin Zhang Bioactive MaterialsVolume 6, Issue 10,October 2021, Pages 3411-3423

      28.Antiepileptic Effects of Cicadae Periostracum on Mice and Its Antiapoptotic Effects in H2O2-Stimulated PC12 Cells via Regulation of PI3K/Akt/Nrf2 Signaling Pathways

      Qing Zhang,Ruo-Lan Li,Ting Tao,Jia-Yi Sun,Jia Liu,Ting Zhang,Wei Peng,Chun-Jie WuVolume2021 29.AGT serves as a potential biomarker and drives tumor progression incolorectal carcinomaWei Chen,Yihuan Chen,Kai Zhang,Wanjing Yang,Xiang Li,Jun Zhao,Kangdong Liu,Ziming Dong,Jing LuInternational Immunopharmacology30.Superlow Dosage of Intrinsically Bioactive Zinc Metal–Organic Frameworks to Modulate Endothelial Cell Morphogenesis and Significantly Rescue Ischemic DiseaseBin Gao,Xiaoyu Wang,Meiyu Wang,Kexin You,Gasim Sebit Ahmed Suleiman,Xiang-Kui Ren,Jintang Guo,Shihai Xia,Wencheng Zhang,Yakai FengACS Nano參考文獻(xiàn)1.Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform應(yīng)用方向：細(xì)胞活力檢測：A549細(xì)胞

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