Synonym:CollagenType4Properties:
RelatedCategories | Adhesion,Attachment,andMatrixFactors,Adhesion,Attachment,andMatrixFactorsforStemCellExpansion,ApplicationIndex,AttachmentFactors,AttachmentsFactors, |
productline | BioReagent |
form | powder |
impurities | HIV,hepatitisBandhepatitisC,nonedetected |
solubility | aceticacid:0.5-2.0 mg/mL(Dissolveforseveralhoursat2-8?°C,occasionallyswirling.) |
suitABIlity | suitableforcellculture |
storagetemp. | −20°C |
GeneInformation | human...COL4A1(1282),COL4A2(1284),COL4A3(1285),COL4A4(1286),COL4A5(1287),COL4A6(1288) |
Description:
Components
Allcollagenmoleculesarecomposedofthreepolypeptidechainsarrangedinatriplehelicalconformation,withaprimarystructurethatismostlyarepeatingmotifwithglycineineverythirdpositionandprolineor4-hydroxyprolinefrequentlyprecedingtheglycineresidue.TypeIVcollagenoccursonlyinthebasementmembranesandcontainsuptosixgeneticallydistincta-chains.
Application
TypeIVcollagenhasbeenfoundtoplayakeyroleinangiogenesis,neurologicaldiseasesandmetastasis.Collagenfromhumanplacentahasbeenusedtoassessthebioelectriceffectsofquinineonairwayepithelialcells,tostudytheselectivetoxicityofengineeredlentviruslyticpeptidesandinaparticleaggregationassayfortherapiddetectionoffibronectin,fibrinogenandcollagenreceptorsonStaphylococcusaureus.Ithasalsobeenusedinamagnesium-dependent,collagen-bindingassayduringcharacterizationofhumanlungtumor-associatedantigens.
Caution
Thisproductshouldbestoreddesiccatedat-20°C,andwillretainactivityintheseconditionsfor3years.
OtherNotes
Collagenisclassifiedintoanumberofstructurallyandgeneticallydistincttypes.WeusethenomenclatureproposedbyBornsteinandTraub.BewaryofconfusingSigma-typedesignationswithrecognizedcollagenclassificationtypes.
PreparationNote
Thisisalyophilizedpowderthatcanbereconstitutedinsterile.5Maceticacid,PBSorwaterat1mg/mL.APBSsolutionwillbestableforatleastoneyearat-20°C.ItwaspreparedbyamodificationofthepepsinextractionmethodofNiyibizi,C.et.al
Biochem/physiolActions
Duringdevelopment,collagenIVisubiquitouslydistributedinBMs.Duringthematurationprocess,thisnetworkgetspartiallyreplacedinaremarkablytissuespecificmanner,definingBMstructureandfunction.CollagenIVhasbeenshowntobindtoplatelets,hepatocytes,keratinocytes,endothelial,mesangial,pancreaticcellsandsometumorcells.
TissueinjuryintheautoimmunediseaseGoodpasturesyndromeisduetopathogenicautoantibodiestargetingtheCollagenIVα3chain.MutationsinCOL4A5areassociatedwithAlportsyndrome.
Safety:
PersonalProtectiveEquipment
Eyeshields,Gloves,typeN95(US),typeP1(EN143)respiratorfilter
RIDADR
NONHforallmodesoftransport