Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The?click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection. The?Cy3?azide is reactive with?terminal alkyne?via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.