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Millipore/AB1956 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr731]/AB1956/100 µL
  • Millipore/AB1956 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr731]/AB1956/100 µL

Millipore/AB1956 | Anti-VE-Cadherin Antibody, phospho-specific [Tyr731]/AB1956/100 µL

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貨號: AB1956
品牌: Millipore
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    • Description
      CatalogueNumberAB1956
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-VE-CadherinAntibody,phospho-specific[Tyr731]
      AlternateNames
      • Cadherin-5
      • Vascularendothelial-cadherin
      • CD144
      BackgroundInformationVE-cadherinisanendothelialspecificadhesionmoleculethatisessentialforthemaintenanceofendothelialbarrierfunctionandangiogenesis.ItislinkedtotheactinCytoskeletonthroughanumberofadaptorproteinsincludingalpha,beta,andgammacatenin.CytoskeletaldynamicsandphosphorylationregulateVE-cadherinmediatedcell-celladhesion.Phosphorylationoftyrosine731leadstotheseparationofbeta-cateninfromthecytoplasmictailofVE-cadherin.Thisphosphorylationeventissufficienttomaintaincellsinamesenchymalstateduringthecellinvasionphaseofangiogenesis.
      ProductInformation
      FormatAffinityPurified
      PresentationAntibodyiscross-adsorbedtononphospho-peptidecorrespondingtothesiteofphosphorylationthenaffinitypurifiedusingphosphorylatedVE-cadherin(Tyr731)peptide.Antibodyissuppliedin100μLphosphate-bufferedsaline(noCa2+,noMg2+),pH7.3,50%glycerol,1mg/mLBSA,and0.05%sodiumazide.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cforuptooneyearfromdateofreceipt.Avoidrepeatedfreeze/thawcycles.Maybestoredforshortperiodsat2-8°C.
      Applications
      ApplicationAnti-VE-CadherinAntibody,phospho-specific[Tyr731]isanantibodyagainstVE-CadherinforuseinWB.
      KeyApplications
      • WesternBlotting
      ApplicationNotesWesternBlot:1:1000.

      Optimalworkingdilutionsmustbedeterminedbytheenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptidecorrespondingtoaminoacidresiduessurroundingandincludingtyrosine731(phosphorylated)ofhumanVE-Cadherin.Theimmunogensequenceis100%identicalinchimpanzee,mouse,rat,chicken,zebrafish,cow,dogand91%identicalinpigVE-cadherin.
      EpitopeTyr731
      HostRabbit
      SpecificityTheantibodydetectsa120kDaproteincorrespondingtoVE-cadherin.
      SpeciesReactivity
      • Human
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisaclassicalcadherinfromthecadherinsuperfamilyandislocatedinasix-cadherinclusterinaregiononthelongarmofchromosome16thatisinvolvedinlossofheterozygosityeventsinbreastandprostatecancer.Theencodedproteinisacalcium-dependentcell-celladhesionglycoproteincomprisedoffiveextracellularcadherinrepeats,atransmembraneregionandahighlyconservedcytoplasmictail.FunctioningasaclassiccadherinbyimpartingtocellstheABIlitytoadhereinahomophilicmanner,theproteinmayplayanimportantroleinendothelialcellbiologythroughcontrolofthecohesionandorganizationoftheintercellularjunctions.Analternativesplicevarianthasbeendescribedbutitsfulllengthsequencehasnotbeendetermined.
      GeneSymbol
      • CDH5
      • VE-cadherin
      • CD144
      • 7B4
      Modifications
      • Phosphorylation
      PurificationMethodImmunoAffinityPurified
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P33151#Cadherinsarecalciumdependentcelladhesionproteins.Theypreferentiallyinteractwiththemselvesinahomophilicmannerinconnectingcells;cadherinsmaythuscontributetothesortingofheterogeneouscelltypes.Thiscadherinmayplayaimportantroleinendothelialcellbiologythroughcontrolofthecohesionandorganizationoftheintercellularjunctions.Itassociateswithalpha-cateninformingalinktothecytoskeleton.
      SIZE:784aminoacids;87516Da
      SUBCELLULARLOCATION:Celljunction.Cellmembrane;Single-passtypeImembraneprotein(Probable).Note=Foundatcell-cellboundariesandprobablyatcell-matrixboundaries.
      TISSUESPECIFICITY:Endothelialtissuesandbrain.
      SIMILARITY:SwissProt:P33151##Contains5cadherindomains.
      MolecularWeight120kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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