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Millipore/AB1623 | Anti-Histone H2B Antibody/AB1623/100 µL
  • Millipore/AB1623 | Anti-Histone H2B Antibody/AB1623/100 µL

Millipore/AB1623 | Anti-Histone H2B Antibody/AB1623/100 µL

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貨號: AB1623
品牌: Millipore
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    • Description
      CatalogueNumberAB1623
      ReplacesMABE15
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionAnti-HistoneH2BAntibody
      ProductInformation
      FormatSerum
      PresentationRabbitantiserum.Liquid.Containsnopreservative.
      StorageandShippingInformation
      StorageConditionsMaintainat-20°Cinundilutedaliquotsforupto12months.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationDetectHistoneH2BalsoknownasHistoneH2BwithAnti-HistoneH2BAntibody(RabbitPolyclonalAntibody),thathasbeendemonstratedtoworkinWB&IHC.
      KeyApplications
      • WesternBlotting
      • Immunohistochemistry
      ApplicationNotesWesternblot

      Immunohistochemistryonparaformaldehydetreatedsections.

      Optimalworkingdilutionsmustbedeterminedbyenduser.
      BIOLOGicalInformation
      ImmunogenSyntheticpeptide
      HostRabbit
      SpecificityReactswithhistoneH2Binhumannuclearfractions.Nocross-reactivitywithotherhistones,H1,H2A,H3orH4.Bindingofhistonesfromotherspecieshasnotbeentested,butitislikelyastheaminoacidsequenceofhistoneH2Bhasbeenfoundtobeidenticalinallspeciesexamined.
      SpeciesReactivity
      • Human
      AntibodyTypePolyclonalAntibody
      EntrezGeneNumber
      EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement.
      GeneSymbol
      • HIST3H2BB
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
      SIZE:126aminoacids;13908Da
      SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
      SUBCELLULARLOCATION:Nucleus.
      PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.
      SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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