The antiserum was produced against synthesized peptide derived from human NFAT3 around the phosphorylation site of Ser168 and Ser170.
Immunogen Range
136-185
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:1000
Specificity
NFAT3 (Phospho-Ser168+Ser170) Antibody detects endogenous levels of NFAT3 only when phosphorylated at Ser168 and Ser170.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFATC4
Database Links
Gene ID: 4776 Swiss-Prot #: Q14934
OMIM #: 602699 Unigene #: Hs.77810
Target Modification
Phospho
Modification Sites
H:S168+S170 M:S168+S170
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NFAT3 (Phospho-Ser168+Ser170) Antibody. The picture on the right is blocked with the phospho peptide.