The antiserum was produced against synthesized peptide derived from human AIRE around the phosphorylation site of Ser156.
Immunogen Range
126-175
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
AIRE (Phospho-Ser156) Antibody detects endogenous levels of AIRE only when phosphorylated at Ser156.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AIRE
Database Links
Gene ID: 326 Swiss-Prot #: O43918
OMIM #: 109100/240300/607358 Unigene #: Hs.129829
Target Modification
Phospho
Modification Sites
H:S156
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with Hu 2nM 24h, using AIRE (Phospho-Ser156) Antibody. The lane on the right is blocked with the phospho peptide.