The antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214.
Immunogen Range
1180-1229
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
VEGFR2 (Phospho-Tyr1214) Antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyr1214.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
KDR
Database Links
Gene ID: 3791 Swiss-Prot #: P35968
OMIM #: 191306 Unigene #: Hs.479756
Target Modification
Phospho
Modification Sites
H:Y1214 M:Y1212 R:Y1210
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is blocked with the phospho peptide.