The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
STAT3 (Phospho-Ser727) Antibody
Synonyms
Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT3 around the phosphorylation site of Ser727.
Immunogen Range
694-743
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
STAT3 (Phospho-Ser727) Antibody detects endogenous levels of STAT3 only when phosphorylated at Ser727.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
STAT3
Database Links
Gene ID: 6774 Swiss-Prot #: P40763
OMIM #: 102582/147060 Unigene #: Hs.463059
Target Modification
Phospho
Modification Sites
H:S727 M:S727 R:S727
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells, using STAT3 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.