The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser33.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
p53 (Phospho-Ser33) Antibody detects endogenous levels of p53 only when phosphorylated at Ser33.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from 293 cells treated with UV, using p53 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.