The antiserum was produced against synthesized peptide derived from human NF-kappaB p100/p52 around the phosphorylation site of Ser869.
Immunogen Range
836-885
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
NF-kappaB p100/p52 (Phospho-Ser869) Antibody detects endogenous levels of NF-kappaB p100/p52 only when phosphorylated at Ser869.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKB2
Database Links
Gene ID: 4791 Swiss-Prot #: Q00653
OMIM #: 164012 Unigene #: Hs.73090
Target Modification
Phospho
Modification Sites
H:S870 M:S869 R:S868
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MDA-MB-435 cells treated with TNF-alpha, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The lane on the left is blocked with the phospho peptide.