The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
LIMK2 (Phospho-Thr505) Antibody
Synonyms
kinase LIMK2; LIM domain kinase 2; LIMK-2
Immunogen
The antiserum was produced against synthesized peptide derived from human LIMK2 around the phosphorylation site of Thr505.
Immunogen Range
471-520
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
LIMK2 (Phospho-Thr505) Antibody detects endogenous levels of LIMK2 only when phosphorylated at Thr505.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
LIMK2
Database Links
Gene ID: 3985 Swiss-Prot #: P53671
OMIM #: 601988 Unigene #: Hs.474596
Target Modification
Phospho
Modification Sites
H:T505 M:T505 R:T505
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using LIMK2 (Phospho-Thr505) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COLO205 cells, using LIMK2 (Phospho-Thr505) Antibody. The lane on the right is blocked with the phospho peptide.