The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23.
Immunogen Range
15-64
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
IKK-alpha (Phospho-Thr23) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at Thr23.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CHUK
Database Links
Gene ID: 1147 Swiss-Prot #: O15111
OMIM #: 600664 Unigene #: Hs.198998
Target Modification
Phospho
Modification Sites
H:T23 M:T23 R:T23
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is blocked with the phospho peptide.