The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
FKHRL1 (Phospho-Ser253) Antibody
Synonyms
AF6q21 protein; FKHR2; FKHRL1; Forkhead box O3; forkhead box O3A; Forkhead box protein O3A; Forkhead in rhabdomyosarcoma-like 1; FOXO3
Immunogen
The antiserum was produced against synthesized peptide derived from human FKHRL1 around the phosphorylation site of Ser253.
Immunogen Range
220-269
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
FKHRL1 (Phospho-Ser253) Antibody detects endogenous levels of FKHRL1 only when phosphorylated at Ser253.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
FOXO3
Database Links
Gene ID: 2309 Swiss-Prot #: O43524
OMIM #: 602681 Unigene #: Hs.220950
Target Modification
Phospho
Modification Sites
H:S253 M:S252
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHRL1 (Phospho-Ser253) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from LOVO cells treated with serum 20% 30', using FKHRL1 (Phospho-Ser253) Antibody. The lane on the right is blocked with the phospho peptide.