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4000-520-616
EnzymeCommission(EC)Number3.2.1.1 ( BRENDA | IUBMB )
MDLnumberMFCD00081319
eCl@ss32160410
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SigmaLifeScienceiscommittedtobringingyou?Greener?AlternativeProducts,whichadheretooneormoreofThe12Principlesof?Greener?Chemistry.Thisproducthasbeenenhancedforwastepreventionwhenusedinstarchethanolresearch.Formoreinformationseethearticleinbiofiles.
Molecularmass:51-54kDa.α-Amylaseisolatedfromporcinepancreasisaglycoprotein.Itisasinglepolypeptidechainof~475residuescontainingtwoSHgroupsandfourdisulfidebridgesandatightlyboundCa2+necessaryforstABIlity.Chlorideionsarenecessaryforactivityandstability.ThepHrangeforactivityis5.5to8.0,withthepHoptimumat7.
Viewmoreinformationonenzymesforcomplexcarbohydrateanalysisatwww.Sigma-Aldrich.com/enzymeexplorerPreparationNote2×crystallizedUnitDefinitionOneunitwillliberate1.0mgofmaltosefromstarchin3minatpH6.9at20°C.PhysicalformSuspensionin2.9MNaClsolutioncontaining3mMCaCl2.Applicationα-Amylaseisusedtohydrolyzeαbondsofα-linkedpolysaccharides,suchasstarchandglycogen.α-Amylase,fromSigma,hasbeenusedinvariousplantstudies,suchasmetabolismstudiesinArabidopsis.Biochem/physiolActionsα-Amylasehydrolyzestheα-(1,4)glucanlinkagesinpolysaccharidesofthreeormoreα-(1,4)linkedD-glucoseunits.Naturalsubstratessuchasstarchandglycogenarebrokendownintoglucoseandmaltose.α-Amylase,fromporcinepancreas,isaglycoproteinthatconsistsofasinglepolypeptidechainofapproximately475residuescontaining2SHgroupsandfourdisulfidebridgesandatightlyboundCa2+necessaryforstability.Safety:Symbol GHS08Signalword DangerHazardstatements H334Precautionarystatements P261-P342+P311PersonalProtectiveEquipment dustmasktypeN95(US),Eyeshields,Faceshields,GlovesRIDADR NONHforallmodesoftransportWGKGermany 1RTECS BU7432500
2×crystallized
Oneunitwillliberate1.0mgofmaltosefromstarchin3minatpH6.9at20°C.
Suspensionin2.9MNaClsolutioncontaining3mMCaCl2.
α-Amylaseisusedtohydrolyzeαbondsofα-linkedpolysaccharides,suchasstarchandglycogen.α-Amylase,fromSigma,hasbeenusedinvariousplantstudies,suchasmetabolismstudiesinArabidopsis.
α-Amylasehydrolyzestheα-(1,4)glucanlinkagesinpolysaccharidesofthreeormoreα-(1,4)linkedD-glucoseunits.Naturalsubstratessuchasstarchandglycogenarebrokendownintoglucoseandmaltose.α-Amylase,fromporcinepancreas,isaglycoproteinthatconsistsofasinglepolypeptidechainofapproximately475residuescontaining2SHgroupsandfourdisulfidebridgesandatightlyboundCa2+necessaryforstability.
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