The antiserum was produced against synthesized peptide derived from human ARHGDIA around the phosphorylation site of Ser174.
Immunogen Range
140-189
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
ARHGDIA (Phospho-Ser174) Antibody detects endogenous levels of ARHGDIA only when phosphorylated at Ser174.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ARHGDIA
Database Links
Gene ID: 396 Swiss-Prot #: P52565
OMIM #: 601925 Unigene #: Hs.159161
Target Modification
Phospho
Modification Sites
H:S174 M:S174 R:S174
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma, using ARHGDIA (Phospho-Ser174) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.