The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
PDCD4 (Phospho-Ser457) Antibody
Synonyms
Programmed cell death protein 4; Nuclear antigen H731-like; Neoplastic transformation inhibitor protein; Protein 197/15a; PDCD4; H731
Immunogen
The antiserum was produced against synthesized peptide derived from human PDCD4 around the phosphorylation site of Ser457.
Immunogen Range
420-469
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
PDCD4 (Phospho-Ser457) Antibody detects endogenous levels of PDCD4 only when phosphorylated at Ser457.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PDCD4
Database Links
Gene ID: 27250 Swiss-Prot #: Q53EL6
OMIM #: 608610 Unigene #: Hs.711490
Target Modification
Phospho
Modification Sites
H:S457 M:S457 R:S457
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PDCD4 (Phospho-Ser457) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with serum 20% 15', using PDCD4 (Phospho-Ser457) Antibody. The lane on the right is blocked with the phospho peptide.