The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254.
Immunogen Range
220-269
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
TNFRSF5 (Phospho-Thr254) Antibody detects endogenous levels of TNFRSF5 only when phosphorylated at Thr254.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD40
Database Links
Gene ID: 958 Swiss-Prot #: P25942
OMIM #: 109535 Unigene #: Hs.472860
Target Modification
Phospho
Modification Sites
H:T254 M:T255
Application Images
Image 1
Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using TNFRSF5 (Phospho-Thr254) Antibody. The lane on the right is blocked with the phospho peptide.