The antiserum was produced against synthesized peptide derived from human RapGEF1 around the phosphorylation site of Tyr504.
Immunogen Range
470-519
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
RapGEF1 (Phospho-Tyr504) Antibody detects endogenous levels of RapGEF1 only when phosphorylated at Tyr504.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RAPGEF1
Database Links
Gene ID: 2889 Swiss-Prot #: Q13905
OMIM #: 600303 Unigene #: Hs.127897
Target Modification
Phospho
Modification Sites
H:Y504
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3nM 40', using RapGEF1 (Phospho-Tyr504) Antibody. The lane on the right is blocked with the phospho peptide.