The antiserum was produced against synthesized peptide derived from human MAD1 around the phosphorylation site of Ser428.
Immunogen Range
394-443
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
MAD1 (Phospho-Ser428) Antibody detects endogenous levels of MAD1 only when phosphorylated at Ser428.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAD1L1
Database Links
Gene ID: 8379 Swiss-Prot #: Q9Y6D9
OMIM #: 602686 Unigene #: Hs.654838
Target Modification
Phospho
Modification Sites
H:S428
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MAD1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.