The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25 around the phosphorylation site of Ser268.
Immunogen Range
223-272
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Specificity
IL-2R alpha/CD25 (Phospho-Ser268) Antibody detects endogenous levels of IL-2R alpha/CD25 only when phosphorylated at Ser268.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IL2RA
Database Links
Gene ID: 3559 Swiss-Prot #: P01589
OMIM #: 147730/601942/606367 Unigene #: Hs.231367
Target Modification
Phospho
Modification Sites
H:S268
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 (Phospho-Ser268) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from LOVO cells, using IL-2R alpha/CD25 (Phospho-Ser268) Antibody. The lane on the right is blocked with the phospho peptide.