The antiserum was produced against synthesized peptide derived from human IL-13R alpha1 around the phosphorylation site of Tyr405.
Immunogen Range
371-420
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Specificity
IL-13R alpha1 (Phospho-Tyr405) Antibody detects endogenous levels of IL-13R alpha1 only when phosphorylated at Tyr405.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
IL13RA1
Database Links
Gene ID: 3597 Swiss-Prot #: P78552
OMIM #: 300119 Unigene #: Hs.496646
Target Modification
Phospho
Modification Sites
H:Y405 M:Y402 R:Y404
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using IL-13R alpha1 (Phospho-Tyr405) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HUVEC cells treated with serum 20% 15', using IL-13R alpha1 (Phospho-Tyr405) Antibody. The lane on the right is blocked with the phospho peptide.