The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser307.
Immunogen Range
273-322
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1000 IF: 1:100~1:1000 ELISA: 1:10000
Specificity
HSF1 (Phospho-Ser307) Antibody detects endogenous levels of HSF1 only when phosphorylated at Ser307.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.