The antiserum was produced against synthesized peptide derived from human hnRNP C1/2 around the phosphorylation site of Ser260.
Immunogen Range
231-280
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
hnRNP C1/2 (Phospho-Ser260) Antibody detects endogenous levels of hnRNP C1/2 only when phosphorylated at Ser260.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human brain, using hnRNP C1/2 (Phospho-Ser260) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with H2O2 100uM 15', using hnRNP C1/2 (Phospho-Ser260) Antibody. The lane on the right is blocked with the phospho peptide.