The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Retinoblastoma (Phospho-Thr826) Antibody
Synonyms
P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein
Immunogen
The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr826.
Immunogen Range
601-650
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Retinoblastoma (Phospho-Thr826) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Thr826.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RB1
Database Links
Gene ID: 5925 Swiss-Prot #: P06400
OMIM #: 109800/180200/259500 Unigene #: Hs.408528
Target Modification
Phospho
Modification Sites
H:T826 M:T819 R:T797
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using Retinoblastoma (Phospho-Thr826) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Retinoblastoma (Phospho-Thr826) Antibody. The lane on the right is blocked with the phospho peptide.