p57 Kip2 (Phospho-Thr310) Antibody detects endogenous levels of p57 Kip2 only when phosphorylated at Thr310.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDKN1C
Database Links
Gene ID: 1028 Swiss-Prot #: P49918
OMIM #: 130650/600856 Unigene #: Hs.106070
Target Modification
Phospho
Modification Sites
H:T310 M:T342
Application Images
Image 1
Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human placenta, using p57 Kip2 (Phospho-Thr310) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15', using p57 Kip2 (Phospho-Thr310) Antibody. The lane on the right is blocked with the phospho peptide.