The antiserum was produced against synthesized peptide derived from human HER3 around the phosphorylation site of Tyr1289.
Immunogen Range
1256-1305
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:10000
Specificity
HER3 (Phospho-Tyr1289) Antibody detects endogenous levels of HER3 only when phosphorylated at Tyr1289.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ERBB3
Database Links
Gene ID: 2065 Swiss-Prot #: P21860
OMIM #: 190151/607598 Unigene #: Hs.118681
Target Modification
Phospho
Modification Sites
H:Y1289 M:Y1286 R:Y1286
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using HER3 (Phospho-Tyr1289) Antibody. The picture on the right is blocked with the phospho peptide.