Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CD28(Phospho-Tyr218)Antibody |
Synonyms | SCFR;Proto-oncogenetyrosine-proteinkinaseKit;c-kit;CD117antigen |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCD28aroundthephosphorylationsiteofTyr218. |
ImmunogenRange | 171-220 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000ELISA:1:40000 |
Specificity | CD28(Phospho-Tyr218)AntibodydetectsendogenouslevelsofCD28onlywhenphosphorylatedatTyr218. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CD28 |
DatabaseLinks | GeneID: 940 Swiss-Prot#: P10747 OMIM#: 186760 Unigene#: Hs.591629 |
TargetModification | Phospho |
ModificationSites | H:Y218M:Y216 |
ApplicationImages |
Image1 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithnocodazole1ug/ml16h,usingCD28(Phospho-Tyr218)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |