The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218.
Immunogen Range
171-220
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
CD28 (Phospho-Tyr218) Antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD28
Database Links
Gene ID: 940 Swiss-Prot #: P10747
OMIM #: 186760 Unigene #: Hs.591629
Target Modification
Phospho
Modification Sites
H:Y218 M:Y216
Application Images
Image 1
Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.