The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
14-3-3 zeta/delta (Phospho-Thr232) Antibody
Synonyms
14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta around the phosphorylation site of Thr232.
Immunogen Range
196-245
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
14-3-3 zeta/delta (Phospho-Thr232) Antibody detects endogenous levels of 14-3-3 zeta/delta only when phosphorylated at Thr232.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
YWHAZ
Database Links
Gene ID: 7534 Swiss-Prot #: P63104
OMIM #: 601288 Unigene #: Hs.492407/Hs.594673
Target Modification
Phospho
Modification Sites
H:T232 M:T232 R:T232
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.