The antiserum was produced against synthesized peptide derived from human PLC beta3 around the phosphorylation site of Ser537.
Immunogen Range
503-552
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
PLC beta3 (Phospho-Ser537) Antibody detects endogenous levels of PLC beta3 only when phosphorylated at Ser537.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PLCB3
Database Links
Gene ID: 5331 Swiss-Prot #: Q01970
OMIM #: 600230 Unigene #: Hs.591953
Target Modification
Phospho
Modification Sites
H:S537 M:S537 R:S535
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PLC beta3 (Phospho-Ser537) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with UV 15', using PLC beta3 (Phospho-Ser537) Antibody. The lane on the right is blocked with the phospho peptide.