The antiserum was produced against synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser147.
Immunogen Range
121-170
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Cyclin B1 (Phospho-Ser147) Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser147.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CCNB1
Database Links
Gene ID: 891 Swiss-Prot #: P14635
OMIM #: 123836 Unigene #: Hs.23960
Target Modification
Phospho
Modification Sites
H:S147 M:S144 R:S137
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human placenta, using Cyclin B1 (Phospho-Ser147) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with UV 15', using Cyclin B1 (Phospho-Ser147) Antibody. The lane on the right is blocked with the phospho peptide.