The antiserum was produced against synthesized peptide derived from human c-Abl around the phosphorylation site of Tyr245.
Immunogen Range
196-245
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000
Specificity
c-Abl (Phospho-Tyr245) Antibody detects endogenous levels of c-Abl only when phosphorylated at Tyr245.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ABL1
Database Links
Gene ID: 25 Swiss-Prot #: P00519
OMIM #: 189980/608232 Unigene #: Hs.431048
Target Modification
Phospho
Modification Sites
H:Y245 M:Y245 R:Y245
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells treated with Insulin 0.01U/ml 15, using c-Abl (Phospho-Tyr245) Antibody. The lane on the right is blocked with the phospho peptide.