The antiserum was produced against synthesized peptide derived from human PAK1 around the phosphorylation site of Thr212.
Immunogen Range
178-227
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
PAK1 (Phospho-Thr212) Antibody detects endogenous levels of PAK1 only when phosphorylated at Thr212.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PAK1
Database Links
Gene ID: 5058 Swiss-Prot #: Q13153
OMIM #: 602590 Unigene #: Hs.435714
Target Modification
Phospho
Modification Sites
H:T212 M:T212 R:T211
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right is blocked with the phospho peptide.