The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853.
Immunogen Range
621-670
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
MYPT1 (Phospho-Thr853) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Thr853.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PPP1R12A
Database Links
Gene ID: 4659 Swiss-Prot #: O14974
OMIM #: 602021 Unigene #: Hs.49582
Target Modification
Phospho
Modification Sites
H:T853 M:T852 R:T855
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is blocked with the phospho peptide.