The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
AS160 (Phospho-Thr642) Antibody
Synonyms
Akt substrate of 160 kDa; KIAA0603; TBC1 domain family member 4; TBC4; TBCD4
Immunogen
The antiserum was produced against synthesized peptide derived from human AS160 around the phosphorylation site of Thr642.
Immunogen Range
611-660
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity
AS160 (Phospho-Thr642) Antibody detects endogenous levels of AS160 only when phosphorylated at Thr642.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TBC1D4
Database Links
Gene ID: 9882 Swiss-Prot #: O60343
OMIM #: 612465 Unigene #: Hs.210891
Target Modification
Phospho
Modification Sites
H:T642 M:T649
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using AS160 (Phospho-Thr642) Antibody. The picture on the right is blocked with the phospho peptide.