The antiserum was produced against synthesized peptide derived from human PYK2 around the phosphorylation site of Tyr580.
Immunogen Range
546-595
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
PYK2 (Phospho-Tyr580) Antibody detects endogenous levels of PYK2 only when phosphorylated at Tyr580.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTK2B
Database Links
Gene ID: 2185 Swiss-Prot #: Q14289
OMIM #: 601212 Unigene #: Hs.491322
Target Modification
Phospho
Modification Sites
H:Y580 M:Y580 R:Y580
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells, using PYK2 (Phospho-Tyr580) Antibody. The lane on the right is blocked with the phospho peptide.