The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Smad3 (Phospho-Ser425) Antibody
Synonyms
JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.
Immunogen Range
376-425
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
Smad3 (Phospho-Ser425) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser425.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 (Phospho-Ser425) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells treated with IGF-I 100ng/ml 10', using Smad3 (Phospho-Ser425) Antibody. The lane on the right is blocked with the phospho peptide.