The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Shc (Phospho-Tyr427) Antibody
Synonyms
SH2 domain protein C1; SHC, SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1
Immunogen
The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427.
Immunogen Range
393-442
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Shc (Phospho-Tyr427) Antibody detects endogenous levels of Shc only when phosphorylated at Tyr427.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SHC1
Database Links
Gene ID: 6464 Swiss-Prot #: P29353
OMIM #: 600560 Unigene #: Hs.433795
Target Modification
Phospho
Modification Sites
H:Y427 M:Y423 R:Y313
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide.