Properties:
RelatedCategories | Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHPLCColumns,TOYOPEARLBulkMedia&Accessories,ToyoScreenAffinityProcessDevelopmentColumns, |
material | aluminumseal |
| polypropyleneandpolyethylenefrit |
packaging | pkgof1×5 mL |
mfr.no. | Tosoh,23431 |
parameter | 1-5mL/minflowrate |
| 20mL/minmax.flowrate |
| 3barmax.pressure |
L×I.D. | 3 cm×14.6 mm |
matrix | hydroxylatedmethacrylicpolymer |
matrixactivegroup | rProteinAphase |
particlesize | 45 μm |
operatingpH | 2-13 |
capacity | >70 g/Ladsorptioncapacity(IgG)(dynamicat5min.residencetime) |
| >80 g/Ladsorptioncapacity(IgG)(static) |
modeofchromatography | Affinity |
Description:
Generaldescription
Everylargescalecommercialpurificationbeginsatasmallscaleinamethoddevelopmentlabbyevaluatingretentionandselectivityofthetargetproteinversusimpurities,hostcellproteins,DNA,etc.,onmultipleresins,oftenfromdifferentvendors.Dependingontheconstraints,theprocessdevelopmentengineercaneitherpurchasebulkresinstopackseveralsmallcolumnswithdifferentresinsortakeadvantageofthespeedandconvenienceofpre-packedcolumnsforresinevaluation.Pre-packedToyoScreen®ProcessDevelopmentColumnsareavailablein1mLand5mLvolumespackedwiththemostpopularToyopearl®resins.ToyoScreencolumnscanbeusedtoquicklyestablishoptimumelutionconditions.VaryingpH,salttype,grADIentconditionsandflowratearecommonexperimentalparametersexplored.InitialresultsfromresinscreeningandoptimizationwithToyoScreencolumnsaccuratelypredicttheseparationbehavioratlargerscale.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
Specifications
Cleaninplacewith0.1MNaOH.
LegalInformation
ToyoScreenisaregisteredtrademarkofTosohCorporation
ToyopearlisaregisteredtrademarkofTosohCorporation