Synonym:
TSKgel® Amide-80, 15 cm x 3 mm ID, 3 μm
Properties:
| Related Categories |
3 μm TSKgel Amide-80 Columns,
Analytical/Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
TSKgel HILIC HPLC Columns,
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material
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stainless steel
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mfr. no.
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Tosoh, 22852
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parameter
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0-100% organic solvent
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10-50 °C temperature
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200 bar max. pressure
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L × I.D.
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15 cm × 3 mm
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matrix
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spherical silica
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matrix active group
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carbamoyl phase
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particle size
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3 μm
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pore size
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100 ?
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surface area
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450 m2/g
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operating pH
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2.0 - 7.5
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suitability
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suitable for L68 per USP
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mode of chromatography
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Hydrophilic interaction (HILIC)
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Description:
General description
TSKgel® Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 ?°C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, high efficiency 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.
Physical form
Shipped in 85% acetonitrile - 15% water.
Recommended products
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Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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