Properties:
RelatedCategories | 3μmTSKgelNH2-100Columns,Analytical/Chromatography,HPLC,HPLC&FPLCColumnsforBiopolymerSeparations,TSKgelHILICHPLCColumns, |
material | stainlesssteelhardware |
mfr.no. | Tosoh,21969 |
parameter | 0-100%organicsolvent |
| 1.0mL/minstandardflowrate |
| 10-50°Ctemperature |
| 50barmax.pressure |
application(s) | HPLC:suitable |
L×I.D. | 5 cm×4.6 mm |
matrix | silicaparticleplatform |
matrixactivegroup | aminephase |
particlesize | 3 μm |
poresize | 100 ? |
surfacearea | 450 m2/g |
operatingpH | 2.0-7.5 |
suitABIlity | suitableforL8perUSP |
modeofchromatography | Anionexchange |
| Hydrophilicinteraction(HILIC) |
| Normalphase |
Description:
Physicalform
Shippedin85%acetonitrile-15%water.
Generaldescription
TSKgelNH2-100aminocolumnsexpandtherangeofTSKgelcolumnsforhydrophilicinteractionchromatography(HILIC).OfferingadifferentselectivityfromthewellknownTSKgelAmide-80columns,thesenovelamino-bondedphasecolumnsstandoutbyprovidingmuchimprovedchemicalstability,aprerequisiteforachievingreproducIBLeandreliableresults.TSKgelNH2-100columnsarepackedwith3micronsilicaparticlescontaining100?pores.Anovelbondingstrategywasadoptedtoimprovechemicalstabilityofthebondedphase.Firstthesilicaisreactedwithatrimethylsilaneendcappingreagentatalowstoichiometricratio,beforereactingresidualandaccessiblesilanolgroupswithtrifunctionalalkylaminosilanereagent.Theresultingbondedphaseprovidesabettersafeguardagainsthydrolysisoftheunderlyingsilica.TSKgelNH2-100columnsareuniqueinthatthebondedphaseligandnotonly,asexpected,hasaterminalprimaryaminogroup,butthatthespaceralsoincorporatessecondaryaswellastertiaryaminogroups.Anioniccompoundsareretainedonthecolumnbyionicinteraction.ThisallowsfortheuseofgrADIentsinsaltconcentration,inadditiontogradientelutionswithacetonitrile.SincetheTSKgelNH2-100columnshavecationicsites,thecolumnscanbeusedasmixedmodecolumnsundersomeconditions.TSKgelNH2-100columnsarewellsuitedfortheanalysisofalltypesofhydrophiliccompounds,includingcarbohydratesandpeptides.Duetoahighliganddensityandlargesurfacearea,thesecolumnsshowstrongerretentionofpolarcompoundsthanTSKgelAmide-80columns.
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LegalInformation
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