Synonym:
TSKgel® Ether-5PW HPLC Column
Properties:
| Related Categories |
Analytical/Chromatography,
HPLC,
HPLC & FPLC Columns for Biopolymer Separations,
Hydrophobic Interaction (HIC) Columns,
TSKgel Ether-5PW Column (1000 ? pore size),
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mfr. no.
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Tosoh, 08641
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parameter
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≤4 M salt concentration
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≤50% organic solvent
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1.2 mL/min flow rate
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10-50 °C temperature
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20 bar max. pressure
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application(s)
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HPLC: suitable
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L × I.D.
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7.5 cm × 7.5 mm
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matrix
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polymer particle platform
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matrix active group
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ether phase
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particle size
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10 μm
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pore size
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1000 ?
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operating pH
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2 - 12
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mode of chromatography
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Hydrophobic interaction (HIC)
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Description:
Physical form
Shipped in water.
General description
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000 ?‚??pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2??M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.TSKgel Ether-5PW, Phenyl-5PW, and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a very hydrophobic packing (e.g., phenyl in this case). We recommend an Ether-5PW column for purifying hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000 ??pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing rapid analyses. All three column types can be cleaned with 0.2?M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
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Legal Information
TSKgel is a registered trademark of Tosoh Corporation
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