Fluorescent IEF Markers,
IEF Standards,
Isoelectric Focusing and Two Dimensional Electrophoresis,
Molecular Biology,
Physical Properties Standards,
Protein Electrophoresis,
Proteomics,
Stock Solution
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concentration
3 mg/mL in H2O
fluorescence
λex 340 nm; λem 420 nm in 0.1 M Tris pH 8.1
storage temp.
2-8°C
Description:
Application
suitable as IEF-Marker for HPCE and for IEF-gels
General description
filtered through a 0.45 μm membrane filter
Reconstitution
For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1μl per lane).