Immunogen:
FractionofbovinebrainGABA-AreceptoraffinitypurifiedagainstimmobilizedbenzodiazepineRo7-1986/1(1988deBlasetalJNeurosciPMID2828565,1988VitoricaetalJNeurosciPMID2828566).TargetinitiallyidentifiedasaGABA-Areceptorby:-ratbrainimmunocytochemistrystainingpatternvialightmicroscopyidenticaltothatseenvialigandautorADIographywith3H-muscimol
-immunoprecipitationof3H-muscimol-bindingactivityofthepurifiedreceptorcomplex
-recognitionof~57kDaproteininimmunoblotsagainstcruderatbrainmembranefractions
Bindingepitopeidentifiedtobewithinaminoacids26-40(QSVNDPGNMSFVKET,extracellularN-terminus,1992EwertetalBrainRes,PMID1377081)ofhumanGABA-A-R-Beta3(alsoknownasGamma-aminobutyricacidreceptorsubunitbeta-3,accessionnumberP28472)
Mouse:100%identity(15/15aminoacidsidentical)
Rat:100%identity(15/15aminoacidsidentical)
86%identity(13/15aminoacidsidentical,QSVNDPSNMSLVKET)withGABA-A-R-Beta2
66%identity(10/15aminoacidsidentical,HSTNEPSNMSYVKET)withGABA-A-R-Beta1
HybridomakindlydonatedbyAngeldeBlas,UniversityofConnecticut.