BlueemittingCoumarin343dyecanformaFRETpairwithfluorescein(FAM).Anaminereactiveform,activatedNHSester.
Thisamine-reactiveNHSestercontainsanaminohexanoyllinkerbetweenthefluorophore,andthereactivegroup.Thislinkerprovidesbettersolubilityandspatialseparationbetweenthefluorophore,andthetargetmoleculebeinglabeled.
ThisreagentcanbeusedtodesignFRETbasedassayswithFAMasacceptor,andtoconstructsystemswhichharvestbluelightenergy.
Generalproperties
Appearance: | yellowsolid |
Molecularweight: | 495.52 |
Molecularformula: | C26H29N3O7 |
Solubility: | goodinDCM,DMSO,DMF |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:12monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |
Spectralproperties
Excitationmaximum,nm: | 437 |
ε,L⋅mol−1⋅cm−1: | 44000 |
Emissionmaximum,nm: | 477 |
Fluorescencequantumyield: | 0.63 |
CF260: | 0.29 |
CF280: | 0.24 |