GoldBio/SHH, Human/1000 μg/6110-19-1000

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Sonic HedgeHogrHuShhHhg2

Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related and share a high degree of amino acid sequence identity. Each Hh protein has a unique expression pattern and distinct biological role within its respective region of secretion.All Hh proteins use the same signaling pathway and can substitute for each other in experimental systems. Shh has been found to have a critical role in development, acting as a morphogen in the patterning of limbs, brain structure, spinal cord, thalamus and the teeth.Shh has been shown to attract commissural axons in the developing spinal cord by attracting or repelling retinal ganglion cell (RGC) axons depending on concentration.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: IVIGPGRGFGKRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQRCKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFDWVYYESKAHI HCSVKAENSV AAKSGG

MW: ~20.0 kDa (176 amino acid)

Purity: >98 % bySDS-PAGE and HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than1.0 μg/ml, corresponding to a specific activity of >1.0 × 10³ IU/mg.

Accession Number: Q15465

HGNC ID: 10848

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80°C for up to 6 months.Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using PBS that contains a 0.1% BSA to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C.Stock solutions should be aliquoted and stored at -80°C. Further dilutionsshould be made in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!