Properties:
| Related Categories |
5 micron LC-Si silica Columns,
Analytical/Chromatography,
HPLC,
HPLC Columns, by Brand for Small Molecules,
LC-Si silica,
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parameter
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≤70 °C temp. limit
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L × I.D.
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30 cm × 4 mm
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matrix
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silica gel, spherical particle platform
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matrix active group
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silica phase
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particle size
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5 μm
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pore size
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120 ? pore size
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surface area
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170 m2/g
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pH-range
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2 - 7.5
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suitability
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suitable for L3 per USP
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compatibility
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mode of use normal phase HPLC
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mode of chromatography
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Hydrophilic interaction (HILIC)
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Normal phase
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Description:
General description
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.
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Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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