Properties:
| Related Categories |
Analytical Column, 3 μm,
Analytical/Chromatography,
Discovery HPLC Columns,
Discovery HS F5,
HPLC,
|
|
feature
|
endcapped
|
|
extent of labeling
|
12% Carbon loading
|
|
parameter
|
≤70 °C temp. range
|
|
application(s)
|
HPLC: suitable
|
|
L × I.D.
|
10 cm × 4 mm
|
|
surface coverage
|
4 μmol/m2
|
|
impurities
|
<10 ppm metals
|
|
matrix
|
silica gel, high purity, spherical particle platform
|
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matrix active group
|
PFP (pentafluorophenyl) phase
|
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particle size
|
3 μm
|
|
pore size
|
120 ?
|
|
surface area
|
300 m2/g
|
|
operating pH range
|
2 - 8
|
|
suitability
|
suitable for L43 per USP
|
|
mode of chromatography
|
Hydrophilic interaction (HILIC)
|
|
|
Reversed phase
|
Description:
Features and Benefits
• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log?Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
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