Properties:
RelatedCategories | AminoHPLCColumns,Analytical/Chromatography,HPLC,HPLCColumns,byBrandforSmallMolecules,HPLCColumns,byPhase, |
packaging | pkgof1 ea |
application(s) | HPLC:suitable |
L×I.D. | 25 cm×10 mm |
matrixactivegroup | aminophase |
particlesize | 5 μm |
poresize | 300 ? |
suitABIlity | suitableforL82perUSP |
modeofchromatography | Hydrophilicinteraction(HILIC) |
| Normalphase |
Description:
Generaldescription
apHera™aminocolumnsarebasedoncovalentlybondedpolyaminespecificallyoptimizedfortheseparationofmono-andoligosaccharides.Theelutionordermono-,di-,tri-saccharideshowsincreasedelutonvolumewithincreasedacetonitrileconcentrationandcompletestabilityforbothacidicandalkalineeluates.Thesmall,robustPVAcopolymerbeadprovidesmechanicalandchemicalstrengthaswellashighcolumnefficiency.Conventionalcamioncolumnsbasedonsilicadonotshowlongcolumnlife,perhapsduetohydrolysisofsilicaparticlebythebasicaminogroup.SinceSupelcousesastrongalkalinecompatIBLepolymer,theseproblemsareeliminated.Stableretentiontimeandlongcolumnlifearealsocharacteristicofthecolumn.
FeaturesofAminoapHera™Columns
• Highefficiencyforcarbohydrateanalysis
• Idealforbasicconditions/amphotericdetection
• Highselectivitymono-tooligosaccharides
• pHrange2-13
• 300angstromporesize
• Stable,predictableretention
• Longcolumnlife
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
LegalInformation
apHeraisatrademarkofSigma-AldrichCo.LLC